Antimicrobial and Anti-inflammatory Activity of Thespesia populnea Mediated Nanoparticles in Murine Mastitis Model

Background: Bovine mastitis is a common inflammatory disease where bacterial resistance to antibiotics major concern in its treatment. Nanoparticles have been known overcoming difficulties of treatment therapies including multi drug resistant infections but research on their role limited. Nowadays Green synthesis nanoparticles gaining importance overcome problems associated with chemical synthesis. Therefore, the present study was conducted evaluate ameliorative action Thespesia populnea (T. populnea) mediated synthesized silver and zinc oxide induced mice. Methods: A total 48 mice were divided into six groups: Group I served as control, groups II VI inoculated Staphylococcus aureus (S. aureus) culture for induction. After induction, III, IV, V received T. methanolic extract (TPE), nano particles (TPSN) (»95 nm size), ZnO (TPZN) (» 85 size) Ceftriaxone respectively. Whole blood collected analyze indicators haptoglobin (Hp) C reactive protein (CRP). The mammary gland weights, load changes tissue samples studied. Result: formation brown colour solution white crystalline precipitate indicated TPE sizes these TPSN TPZN when characterized by SEM, found be 95 values weight, load, Hp CRP elevated significantly group compared other groups. Histopathological examination revealed clumps, severe leucocytic infiltration along congestion necrotic II. ameliorating damaged tissues experimental remained decreasing order TPSN, ceftriaxone, phytogenic can effectively used control mastitis.